Bioassay of Human Granulocyte Colony-Stimulating Factor Using Human Promyelocytic HL-60 Cells.
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منابع مشابه
Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris
Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 1997
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.20.943